BIO-DL was established in 2013, located in Songjiang District, Shanghai, China, with an office area of more than 3,000 square meters; it is a comprehensive company integrating R&D, production and trade, and has a worldwide sales network and service centers. BIO-DL is dedicated to offering high quality technology products and services, as well as creating pleasant experiences for the users. BIO-DL has passed CE certification, ISO9001 quality management system certification and ISO13485 medical device quality management system certification. In addition, we have 2 software copyrights and 7 utility model patents.
Product Introduction of Full Wavelength and High-Throughput UV-Vis Nucleic Acid Microplate Reader
The working principle of the full wavelength enzyme-linked immunosorbent assay (ELISA) is mainly based on the Lambert Beer law and spectral analysis techniques. Here is its specific working principle:
The light source emits composite light: The full wavelength enzyme-linked immunosorbent assay (ELISA) analyzer has a light source that can emit a wide spectrum, such as a xenon lamp, tungsten lamp, or light emitting diode (LED). These light sources can generate composite light covering the range of ultraviolet, visible, and near-infrared, providing a basis for subsequent detection of different wavelengths.
The spectroscopic system obtains monochromatic light: The composite light emitted by the light source is decomposed into monochromatic light of different wavelengths through a spectroscopic system, such as diffraction gratings, filters, or monochromators. In this way, specific wavelengths of light can be selected to irradiate the sample according to experimental requirements, in order to accurately measure the absorbance of the sample at different wavelengths.
Monochromatic light irradiation sample: The monochromatic light obtained through the spectroscopic system is irradiated onto the sample to be tested in the microplate. Microplate is a transparent plastic plate with multiple uniformly sized small holes on top, each containing a sample to be tested. The corresponding antigen or antibody may be pre embedded in the holes to undergo specific reactions with the substance to be tested.
Sample absorption of light: When monochromatic light passes through the sample, the material in the sample absorbs a portion of the light's energy. According to Lambert Beer's law, under certain conditions, the degree of light absorption by a sample is proportional to the concentration of the absorbing substance in the sample. Its expression is, where is the absorbance, is the molar absorptivity, is the optical path length, and is the concentration of the substance.
Photodetector conversion signal: Light that is not absorbed by the sample is transmitted through the sample and then irradiated onto the photodetector. Photodetectors can convert optical signals into electrical signals, and the magnitude of the converted electrical signals varies depending on the intensity of the light, thus achieving the conversion from optical signals to electrical signals.
Signal processing and data analysis: The electrical signals generated by photodetectors are usually relatively weak and require a series of signal processing processes such as preamplification, logarithmic amplification, and analog-to-digital conversion. The processed digital signal is sent to the microprocessor, which processes and calculates the data based on Lambert Beer's law and the built-in algorithm of the instrument, obtaining relevant data such as the absorbance value of the sample, and can further calculate information such as the concentration of the analyte in the sample.
Result display and output: Finally, the processed data will be displayed on the monitor, and the detection results can also be printed out through a printer or transmitted to external devices such as computers for further analysis and storage.
Performance characteristics of Full Wavelength and High-Throughput UV-Vis Nucleic Acid Microplate Reader
Wide wavelength coverage range: It can cover the full wavelength range of 200nm-800nm, which can meet the detection needs of biological macromolecules such as nucleic acids and proteins, as well as various fluorescent dyes, enzyme substrates, etc. in the ultraviolet and visible light regions.
High throughput detection: It can quickly detect 96 well or 384 well microplates. For example, BIO-DL's K3 MAX detection of 96 well plates only takes 6 seconds, and 384 well plates only takes 10 seconds, greatly improving experimental efficiency and suitable for large-scale sample screening and analysis.
High resolution and accuracy: The wavelength resolution can reach 1nm, which can accurately distinguish the changes in absorbance at different wavelengths. The bandwidth is less than 2.5nm, and the stray light is less than 0.01%, ensuring the accuracy and reliability of the detection results.
Wide dynamic range: The absorbance reading range can usually reach 0-3.0OD, which can detect samples from extremely low to high concentrations. The linear range is good, and accurate measurement results can be provided in different absorbance ranges.
Full wavelength enzyme-linked immunosorbent assay (ELISA) has been widely used in many scientific and clinical fields, including:
Molecular Biology: Used for DNA and RNA concentration measurement, PCR reaction monitoring, etc.
Immunology: Used for ELISA, protein quantification, cell proliferation and other experiments.
Pharmaceutical industry: used for drug screening, drug metabolism research, etc.
Food and beverage analysis: used for the detection of nutrients and pollutants in food.
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FAQ
Q: How about quality of the goods?
A: All the running products comply with CE standards.
Independent quality inspection team, conducting multiple inspections and random inspections from parts supply to product dispatch.
Q: Is OEM service available.
A: All of the running products are available for OEM customization, including logo and package.
