Ultra micro spectrophotometer has become a routine instrument in modern molecular biology laboratories. Commonly used for nucleic acid, protein quantification, and bacterial growth concentration quantification. Spectrophotometers are highly precise instruments, and it is important to pay attention to the operating techniques during use. Improper operation may affect the experimental results. So, how to correctly operate ultra micro spectrophotometers? Let's take a look together below.
Firstly, before starting the ultra micro spectrophotometer, it is essential to remove the moisture-proof agent stored in the sample room (important thing to say three times), otherwise there will be serious consequences. During the process of testing the sample, do not open the sample chamber cover; It is required that the liquid in the colorimetric dish accounts for 66% -80% of the total volume, and too much liquid should not be allowed to leak and corrode the instrument; During normal testing, it is necessary to ensure that the colorimetric dish is clean, and the droplets on the inner wall need to be wiped with professional paper. Do not use your hands to directly wipe, as it may cause harm to the hands of the user. I believe no one wants this to happen.
When the ultra trace spectrophotometer is operating normally, it is strictly prohibited to place liquid solvents on the surface of the instrument. If there is a leakage of liquid, it is necessary to ensure timely cleaning and treatment. After the detection is completed, it is necessary to promptly dispose of the liquid in the colorimetric dish, clean it with distilled water, and invert it for air drying. Afterwards, the power of the instrument needs to be turned off, the moisture-proof agent should be placed in the sample room, and dust prevention measures should be taken. Only with the approval of the management personnel can the instrument be left.
Microdrop ultra micro spectrophotometer
