In the evolving world of scientific research, precision and minimal sample usage are critical—especially in molecular biology, biochemistry, and life sciences. This is where the UV micro spectrophotometer proves its value. But what exactly is its use?
A UV micro spectrophotometer is a compact, high-precision instrument used to measure the absorbance and concentration of nucleic acids (DNA, RNA), proteins, and other biomolecules in extremely small volumes—typically in the microliter range. Unlike traditional spectrophotometers, it requires no cuvettes or large sample volumes, making it ideal for precious or limited samples.
Key Uses of a UV Micro Spectrophotometer:
Quantification of DNA, RNA, and Protein
It’s widely used to measure nucleic acid purity and concentration based on UV absorbance at 260 nm and 280 nm.
Purity Assessment
The device provides A260/A280 and A260/A230 ratios to assess sample contamination from proteins, salts, or solvents.
Enzyme Kinetics and Reaction Monitoring
Researchers use it to observe real-time changes in absorbance during biochemical reactions.
Minimal Sample Volume Requirement
With as little as 0.5–2 µL of sample needed, it helps conserve precious reagents and biomaterials.
Fast and Accurate Results
Most models provide instant results without lengthy preparation, supporting high-throughput lab workflows.
No Consumables Needed
Many UV micro spectrophotometers use a wipe-clean sample pedestal, eliminating the cost of cuvettes and tips.
In conclusion, the UV micro spectrophotometer is a powerful tool for researchers who need fast, accurate, and low-volume sample analysis. Its applications in genomics, proteomics, and drug discovery make it an indispensable part of modern lab environments. As labs strive for greater efficiency and precision, this device stands out for its convenience, reliability, and performance.
